http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-1797194-A2
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_d5cd84f92e5e2ff95dfd19e4e329db3b |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-005 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-00 |
| filingDate | 2005-10-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a9d7d9a9829e7bb7d32b4041e56e5de2 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_268d3e5a8a423258f79c9724b32dbbd5 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c7e5c1a8dbb0370222c545ef6585fd07 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ad27f89153294525e5b3f24d7e871286 |
| publicationDate | 2007-06-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | EP-1797194-A2 |
| titleOfInvention | Stable three enzyme creatinine biosensor |
| abstract | The invention provides methods for preparing a stable, multiple-use three enzyme biosensor for the amperometric determination of creatinine in biological liquids that has a useful lifetime that extends significantly beyond that of presently available amperometric biosensors. The biosensor prepared by the methods of the invention encompasses a plurality of immobilized enzymes that are applied to the biosensor as an enzyme-polymer composition. The enzymes, which can include creatinine amidohydrolase, creatine amidinohydrolase and sarcosine oxidase, are immobilized into the enzyme-polymer composition simultaneously as well as applied to the biosensor simultaneously. Prior to being immobilized, the enzymes can be chemically modified by attaching one or more polyethylene glycol (PEG) chains per enzyme monomer. The polymer component can be provided by a polyurethane membrane. The invention also provides a method of preparing a biosensor that limits the diffusion of silver ions emanating the reference electrode, thereby preventing, contact between the silver ions and the enzymes. Related methods of preparing an enzyme-polymer composition for incorporation into a multiple use three enzyme biosensor for the amperometric determination of creatinine in biological liquids also are provided. The invention also provides multiple-use biosensors and enzyme-polymer compositions prepared by the methods disclosed. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112881585-A |
| priorityDate | 2004-10-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 342.