http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-1777291-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_f4ead6158fa635796a5fa4d48ffc357a |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1003 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate | 2005-10-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1473362090d28b3b231f7b2bbfbb3aee http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_70bf0de9e61f8d34ae2a52a3fbfb3882 |
| publicationDate | 2007-04-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | EP-1777291-A1 |
| titleOfInvention | Method for the isolation of mRNA from formalin fixed, paraffin-embedded tissue |
| abstract | The invention provides a reliable method for recovering RNA from a formalin-fixed, paraffin-embedded biological tissue sample comprising: na) deparaffinizing the sample, nb) contacting said sample with a solution comprising an effective concentration of proteinase K and heating the sample in said solution to a temperature in the range of about 30 to about 60 °C, for a time period of about 8 to 24 hours, nc) adding an effective concentration of proteinase K to the solution obtained from b) for a time period of about 5 to about 30 minutes at a temperature range of about 40 to about 75 °C, and nd) recovering said RNA from said solution. n The additional step c) of digestion improves significantle the recovery of RNA. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-2054505-A4 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2021198377-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-2054505-A2 |
| priorityDate | 2005-10-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 79.