http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-1762627-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_efda7341819f1a85d7f2fe104cd8a26f |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6806 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6844 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate | 2005-09-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1373a00e11223d5b83c31a4ae3cb23db http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_135f30b72e489f20d4003baa11862aca |
| publicationDate | 2007-03-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | EP-1762627-A1 |
| titleOfInvention | Method for the activation of a nucleic acid for performing a polymerase reaction |
| abstract | The present invention relates to a method for activating a nucleic acid for a strand displacement reaction comprising the steps of: (a) heating a nucleic acid (preferably a double-stranded DNA) to a temperature of 50 to 90 ° C, (b) cooling the nucleic acid a temperature at which a polymerase does not show a significant decrease in activity, and (c) starting the strand displacement reaction by adding a heat labile polymerase to the nucleic acid. In an alternative method employing a heat stable polymerase, the polymerase may be added immediately in step (a). The method can be carried out both with purified nucleic acid and nucleic acid contained in a cell. |
| priorityDate | 2005-09-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 46.