| abstract |
A method for the multiplexed diagnosis of a plurality of different biomolecules in a fluid sample substantially simultaneously is provided. In accordance with a method of the invention, a substantial fraction of biomolecules in a fluid sample are complexed with a universal label and a secondary labeling reagent. Flow cytometric measurements may be used to identify and quantify, in real-time, by detecting the secondary reagent and universal label present in any of said complexes. The inventive technology enables the simultaneous, and automated, detection and interpretation of multiple biomolecules while also reducing the cost of performing diagnostic and genetic assays. |