http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-1731603-B1
Outgoing Links
Predicate | Object |
---|---|
assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_9019c0ce5cb7b82c85e121f6cac86dce |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-5035 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-62 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-62 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-50 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-15 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
filingDate | 2005-03-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2012-05-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_aea56fa6a68fc7f60103efa56453901c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f14c8ccc83e4912cef06453f5d72fde7 |
publicationDate | 2012-05-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | EP-1731603-B1 |
titleOfInvention | Methods of detecting and quantifying protein nuclear migration using pairs of probes |
abstract | A convenient and highly accurate method for detecting protein nuclear migration due to an endogenous or foreign substance in a local area such as a vital cell or an individual organism with the use of a pair of probes for detecting protein nuclear migration, characterized in that the probes comprising a probe I having the protein, the nuclear migration of which is to be detected and quantified, connected to either the N-terminal side or the C-terminal side of a fused structure [intein-C/labeled protein-C] wherein at least a C-terminal side polypeptide obtained by halving intein is connected to a C-terminal side polypeptide obtained by halving a labeled protein in this order, and another probe II having a nuclear localization signal connected to either the N-terminal side or the C-terminal side of a fused structure [labeled protein-N/intein-N] wherein at least the remaining N-terminal side polypeptide obtained by halving intein is connected to the remaining N-terminal side polypeptide obtained by halving the labeled protein in this order. |
priorityDate | 2004-03-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 240.