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filingDate 2006-05-15-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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publicationDate 2006-11-29-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber EP-1726641-A1
titleOfInvention Cell separation method and kit using phase separation
abstract A method of separating cells or viruses from a mixture using phase separation is provided. The method includes: suspending a sample containing cells or viruses in a kosmotropic salt solution having a pH of 3-6; aggregating the cells or viruses by adding a cationic polymer to the suspension; adhering the cells or viruses to a solid substrate with the aid of phase separation of polymer by placing the suspension in contact with the solid substrate; and separating the solid substrate on which the cells or viruses are adhered from the suspension. Accordingly, it is possible to separate a high concentration of cells, even when using a flow control system. In addition, the method can be conveniently applied to lab-on-a-chip technology.
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