| abstract |
The invention relates to a method for production of neurons from cells of a cell line which may be differentiated to produce neurons in particular, whereby said cells are cultivated in spheres, preferably by exposing the same to growth factors, such as, for example, EGF (epidermal growth factor) and/or bFGF (basic fibroblast growth factor) or LIF (Leukemia Inhibitory Factor), in a given growth medium, the differentiation in said spheres is induced on forcing the same to adhere to a substrate, after removal of the growth factors EGF and/or bFGF or LIF, and cultivating the same in the growth medium for an appropriate duration. Said method is characterised in that cells of the human embryonic teratocarcinoma NT2 are used. |