http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-1657548-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_d6a6f422b091ba12ea61d4adbf1b0e8e http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_24324b37f9f894773efbcfcced4e4138 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_f5ffafaf67c9aace9dbacffadf51aa48 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01D59-44 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-48 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C01B4-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N31-12 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-48 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C01B4-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/B01D59-44 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N31-12 |
| filingDate | 2004-08-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c28840faa7a4cdc4b698938a9600ab5b http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6083e1fa3be7e2929520e1cd5e8d02cc http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_fc5010112e62d15cfa1828c4832e9c99 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c10add041ce9d845fabc4ee6a9ea1fd8 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5cb0b92eed2ad306c3e67f18e5eeb3a0 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_27c805bf6e66cacb906df005f9b5fd90 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_be2c1fb342e2283461b5225f7606e462 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1d3ea452f2cedd104bf5f712a4e770fd |
| publicationDate | 2006-05-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | EP-1657548-A1 |
| titleOfInvention | Method of confirming hydrogen/deuterium exchange in constructions of biological samples and determining the percentage of deuterium in biological samples |
| abstract | The invention provides a method for analyzing the hydrogen isotopes, in particular, a method for confirming hydrogen/deuterium exchange in the structure of a biological sample and determining the percent content of deuterium in a biological sample. The method comprises the following steps: selecting a matrix, mixing homogeneously the prepared biological sample with a suitable amount of matrix followed by lyophilization, sufficiently oxidizing the lyophilized mixed sample with oxidants through burning in order to oxidize the hydrogen in the mixed sample to water, reacting the resulting water after separation with zinc to generate hydrogen gas, determining the 2 H/ 1 H ratio of the hydrogen gas with a gas isotope mass spectrometer, and then calculating the percent content of deuterium in the biological sample through a formula. When the percent content of deuterium in a biological sample increases, its biological characters are markedly changed, and mainly, the heat resistance is markedly changed. The percent content of deuterium in a biological sample having optimal heat stability and the optimal deuteration conditions of a biological sample are ascertained, and the stability of biological sample such as polypeptide is improved. This method has a good repeatability of determination, is simple and easy to manipulate, requires a small amount of sample used, and has low cost. |
| priorityDate | 2003-08-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 90.