| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_4eb6dbca25e5f9459a6c5f592728c787 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-485 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-48
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-37
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-48
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-42 |
| filingDate |
2004-07-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate |
2008-09-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_dd0ace900cdd228a20fc7b61e5ae555b
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a0efcabd329759f493f74c7308838a7b
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_71ad4d70f098a58286d2b63fcedb7869
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_fea9650eb663aa3fffd0e5ed14c80832
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8e160d4518f565e8a674b627a402c6d2
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8d394954b6279676cf0d0541aa66d0df |
| publicationDate |
2008-09-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
EP-1644512-B1 |
| titleOfInvention |
Fluorine nmr spectroscopy for biochemical screening |
| abstract |
High-Throughput Screening (HTS) of large compound libraries is the method of drug-lead discovery. It is now well accepted that for a functional assay, quality is more important than quantity. A biochemical NMR method originally proposed by Percival and Withers (Biochemistry, 1992, 31, 498-505) is extended to the screening of Ser/Thr kinases. The method requires the presence of a CF3 (or CF) moiety on the substrate and utilizes 19F NMR spectroscopy for the detection of the starting and enzymatically modified substrates. Experiments can be performed in real time or in an endpoint assay format using protein and substrate concentrations comparable to the ones used by other HTS techniques. Application of this technique to the phosphorylation of a substrate by the protein Ser/Thr kinase AKT1 is presented. |
| priorityDate |
2003-07-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |