| abstract |
The invention relates to a process for the biosynthetic production ofn4-amino-4-deoxychorismate (ADC) performed fermentatively in vivo with an4-amino-4-deoxychorismate synthase, preferably a PabAB bipartite protein (whichnmay be a fusion protein), at an increased level of activity, thereby obtaining a brothncomprising ADC and 4-amino-4-deoxyprephenate (ADP), that are recovered. Theninvention also relates to a further process of converting the ADP intonp-aminophenylalanine. The invention, moreover relates to biosynthetic production ofn[3 R ,4 R ]-4-amino-3-hydroxycyclohexa-1,5-diene-1-carboxylic acid (3,4-CHA), bynconcerted action of such 4-amino-4-deoxychorismate synthase and of an enzymencapable of converting isochorismate into [5 S ,6 S ]-5,6-dihydroxycyclohexa-1,3-diene-1-carboxylicnacid (2,3-CHD), preferably a phenazine biosynthesis protein PhzD,nincluding recovery of 3,4-CHA. The invention also relates to expression vectors andnhost cells for use in any of such processes. And the invention finally relates tonsynthesis of oseltamivir phosphate from 3,4-CHA. |