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filingDate 1998-08-05-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_cb76fdd273533d29df5a88acfbefb9fa
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4e5ab8fa50c841c00ad0c02543da5f17
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publicationDate 2005-08-31-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber EP-1568787-A1
titleOfInvention Nucleic acid sequences that can be used as primers and probes in the amplification and detection of all subtypes of HIV-1
abstract The present invention is related to nucleic acid sequences that can be used in the field ofnvirus diagnostics, more specifically the diagnosis of infections with the AIDS causing HumannImmuno-deficiency Virus (HIV). n With the present invention nucleotide sequences are provided that can be used as primersnand probes in the amplification and detection of HIV-1 nucleic acid. The oligonucleotidensequences provided with the present invention are located in the LTR part of the HIV viralngenome. It has been found that, by using the sequences of the present invention in methodsnfor the amplification and detection of nucleic acid a sensitive and specific detection of HIV-1ncan be obtained. The benefit of the sequences of the present invention primarily resides innthe fact that, with the aid of primers and probes comprising the sequences according to theninvention the nucleic acid of all presently known subtypes of HIV-1 can be detected with highnaccuracy and sensitivity. So far no primer pairs or hybridization probes have been developednthat would allow the detection of such a broad range of HIV-1 variants. n The oligonucleotide sequences according to the present invention are especially useful innmethods for the amplification of nucleic acid.
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