| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_7c3abccac7bae9414dc8ce09b2d192dc |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-88
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P13-004
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-52 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K19-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-88
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-60
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-52
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P13-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-04 |
| filingDate |
2001-12-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate |
2009-02-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4ce17ea5cbdf95d9ae643cb1a4b395d4
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_757b0308f2d9ed46c736f67df3265a19
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_080deb01b267affa9c639b6d239fbb2e
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9a98670a083304e9ab4c6827712349ee
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_461b6022e4d712c1d0455f7b082bd95c
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a4791da6713ebf148ea4d4e6080f98e0
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_67a89796f3c489f00c025f583b671cc7
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8869103d7ec31f7aab3ce754af32a216
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ec47537d25e913042f0c1f99c282e2cc
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_293fcd20493a6b45ca6eeb4ea97ca0cb |
| publicationDate |
2009-02-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
EP-1566441-B1 |
| titleOfInvention |
Genes coding for hydroxynitrile lyase, recombinant proteins with hydroxynitrile lyase activity and their use |
| abstract |
Gene (I) containing a DNA sequence that encodes a hydroxynitrile lyase (HNL) is new. It is prepared using a combination of primers based on the 5' and 3' regions of the MDL1 gene of Prunus amygdalus and one of the mdl1-5 genes of P. serotina, by standard amplification of DNA from organisms that contain HNL-encoding genes, then cloning. Independent claims are also included for: (1) recombinant protein (II) produced by heterologous expression of the new HNL gene sequences from P. amygdalus in a host cell; (2) fusion protein (FP) with HNL activity prepared using (I) and a signal sequence for secretory expression in a host cell; and (3) method for preparing (S)- or (R)-cyanohydrins (II) by reacting an aliphatic or (hetero)aromatic aldehyde or ketone with a cyanide donor in presence of (II) or FP, in organic, aqueous or two-phase systems, or in an emulsion. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8846366-B2 |
| priorityDate |
2001-01-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |