http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-1553187-A1

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assignee http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_9019c0ce5cb7b82c85e121f6cac86dce
classificationCPCInventive http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-26
classificationIPCInventive http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-26
filingDate 2003-05-08-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_633016a5572d36859dbab1eeb53d61ed
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f24a7b7871f17c5aa6bc5ef0ba144ae1
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_689414d1d46c4081cbfb670652c569d1
publicationDate 2005-07-13-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber EP-1553187-A1
titleOfInvention Method of scavenging intermediate formed by reaction of oxidoreductase with substrate
abstract The present invention is the method to trap reliably the reaction intermediatesnof an oxidoreductase, such as cytochrome-c oxidase. n The present invention is a method for trapping reaction intermediates of annoxidoreductase comprising the steps of:n (the first step) dissolving an oxidoreductase, a photoinduced reducing agent thatnreleases electrons by light-irradiation, amine-type electron donor and a substrate fornsaid oxidoreductase in water and mixing these; (the second step) cooling the mixture prepared in the first step to 70∼270K to benfrozen; (the third step) irradiating the frozen mixture prepared in the second step at 70∼270Knwith a light in a wavelength region including the absorbing wavelength of saidnphotoinduced reducing agent; and (the fourth step) raising the temperature of the frozen mixture prepared in the thirdnstep to the temperature of 80∼270K that is higher than the temperature of the thirdnstep.
priorityDate 2002-08-28-04:00^^<http://www.w3.org/2001/XMLSchema#date>
type http://data.epo.org/linked-data/def/patent/Publication

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http://rdf.ncbi.nlm.nih.gov/pubchem/protein/ACCP00432
http://rdf.ncbi.nlm.nih.gov/pubchem/protein/ACCP14675
http://rdf.ncbi.nlm.nih.gov/pubchem/gene/GID820582
http://rdf.ncbi.nlm.nih.gov/pubchem/protein/ACCQ0ITW7
http://rdf.ncbi.nlm.nih.gov/pubchem/protein/ACCP14674
http://rdf.ncbi.nlm.nih.gov/pubchem/protein/ACCB4M8Y0
http://rdf.ncbi.nlm.nih.gov/pubchem/protein/ACCQ2FYU7
http://rdf.ncbi.nlm.nih.gov/pubchem/gene/GID833833
http://rdf.ncbi.nlm.nih.gov/pubchem/protein/ACCP25890
http://rdf.ncbi.nlm.nih.gov/pubchem/protein/ACCO68146
http://rdf.ncbi.nlm.nih.gov/pubchem/protein/ACCP80222
http://rdf.ncbi.nlm.nih.gov/pubchem/protein/ACCQ64413
http://rdf.ncbi.nlm.nih.gov/pubchem/protein/ACCQ8CQ56
http://rdf.ncbi.nlm.nih.gov/pubchem/gene/GID817626
http://rdf.ncbi.nlm.nih.gov/pubchem/protein/ACCQ64405
http://rdf.ncbi.nlm.nih.gov/pubchem/gene/GID32444
http://rdf.ncbi.nlm.nih.gov/pubchem/gene/GID843795
http://rdf.ncbi.nlm.nih.gov/pubchem/compound/CID1474
http://rdf.ncbi.nlm.nih.gov/pubchem/protein/ACCQ55DH8
http://rdf.ncbi.nlm.nih.gov/pubchem/gene/GID4984247
http://rdf.ncbi.nlm.nih.gov/pubchem/protein/ACCQ64415

Total number of triples: 665.