http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-1553187-A1
Outgoing Links
Predicate | Object |
---|---|
assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_9019c0ce5cb7b82c85e121f6cac86dce |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-26 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-26 |
filingDate | 2003-05-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_633016a5572d36859dbab1eeb53d61ed http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f24a7b7871f17c5aa6bc5ef0ba144ae1 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_689414d1d46c4081cbfb670652c569d1 |
publicationDate | 2005-07-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | EP-1553187-A1 |
titleOfInvention | Method of scavenging intermediate formed by reaction of oxidoreductase with substrate |
abstract | The present invention is the method to trap reliably the reaction intermediatesnof an oxidoreductase, such as cytochrome-c oxidase. n The present invention is a method for trapping reaction intermediates of annoxidoreductase comprising the steps of:n (the first step) dissolving an oxidoreductase, a photoinduced reducing agent thatnreleases electrons by light-irradiation, amine-type electron donor and a substrate fornsaid oxidoreductase in water and mixing these; (the second step) cooling the mixture prepared in the first step to 70∼270K to benfrozen; (the third step) irradiating the frozen mixture prepared in the second step at 70∼270Knwith a light in a wavelength region including the absorbing wavelength of saidnphotoinduced reducing agent; and (the fourth step) raising the temperature of the frozen mixture prepared in the thirdnstep to the temperature of 80∼270K that is higher than the temperature of the thirdnstep. |
priorityDate | 2002-08-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 665.