| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_55bc2bae9f65cfaede67b62128f00e9b |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C40B40-06
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07B2200-11
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y02P20-55 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C40B50-14
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07H21-04
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07H19-20
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07H21-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07H19-10 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H19-10
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H19-20
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H21-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07B61-00 |
| filingDate |
1999-08-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6301ac4c4efa242844a0cc817ac0550e
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6889852d277d79eea8feba38ac15e1f1
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6bec817adf655a95c4547714c50446c7 |
| publicationDate |
2005-07-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
EP-1553102-A1 |
| titleOfInvention |
Synthesis of oligonucleotides |
| abstract |
The invention provides a method for synthesizing oligonucleotides using carbonatenprotection of hydroxyl groups and nucleophilic deprotection reagents. The deprotection reagentsnirreversibly cleave the carbonate protecting groups while simultaneously oxidizing thenintemucleotide phosphite triester linkage, and can be used in aqueous solution at neutral to mildlynbasic pH. The method eliminates the need for separate deprotection and oxidation steps, and,nsince the use of acid to remove protecting groups is unnecessary, acid-induced depurination isnavoided. Fluorescent or other readily detectable carbonate protecting groups can be used,nenabling monitoring of individual reaction steps during oligonucleotide synthesis. The inventionnis particularly useful in the highly parallel, microscale synthesis of oligonucleotides. Reagentsnand kits for carrying out the aforementioned method are provided as well. |
| priorityDate |
1998-08-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |