http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-1546328-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_69c68a76c0f543aff42aff4f02b319c5 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2800-30 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2840-20 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2830-60 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2830-15 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2830-00 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-85 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-907 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-85 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-90 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-10 |
| filingDate | 2003-10-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_15b953dc7b14dc2b5b664ac9f7ed3c09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_71f0deeab0d6230a1f049be739fedee3 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8956de9c4a13a4e60bfd8be4de3af858 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_55e0bd586c4454c8427ea10a6a10f019 |
| publicationDate | 2005-06-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | EP-1546328-A1 |
| titleOfInvention | High yield heterologous expression cell lines for expression of gene products with human glycosylation pattern |
| abstract | Preparing a cell capable of stable high yield expression of a target gene product comprising selecting a starting cell capable of stable high yield expression of a starting gene product (SGP) non-essential to the starting cell, screening for the locus of the SGP within the cell, and replacing the gene coding for the SGP with a DNA sequence containing one or more recombinase recognition sites to obtain a functionalized precursor cell, is new. Preparing a cell capable of stable high yield expression of a target gene product comprising selecting a starting cell capable of stable high yield expression of a starting gene product being non-essential to the starting cell, screening for the locus of the starting gene product within the genome of the starting cell, and replacing the gene coding for the starting gene product with a first functional DNA sequence containing one or more recombinase recognition sites (RRS) to obtain a functionalized precursor cell, is new. The method further comprises integrating a second functional DNA sequence containing a DNA sequence coding for the target gene product into the functionalized precursor cell obtained by use of recombinase recognizing the RRSs incorporated with the first functional sequence. The replacement step alternatively comprises directly replacing the gene coding for the starting gene product with a functional DNA sequence containing a DNA sequence coding for the target gene product. Independent claims are also included for: (1) a cell capable of high yield expression of a target gene product obtainable by the method cited above; (2) preparing a functionalized cell comprising the steps of the method cited above; (3) a functionalized cell by the method of (2); and (4) high yield expression of a target gene product which comprises cultivating a cell of (1). |
| priorityDate | 2002-10-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 348.