| abstract |
The present invention provides a scaffold in whichncells can be stably retained and grafted in a uniformndistribution state in the culture, preferable proliferationnability and viability can be secured, and particularly innthe case of cartilage, fixation treatment such as suturencan be carried out in the transplantation into affectednparts after the culture, and the mechanical strength isnprovided sustainable for (weighted) compression at theninitial stage of transplantation. n The present invention relates to a 3-dimensionalnporous scaffold for tissue regeneration n which comprises a structure composed of verticallynlong-shaped pores having a pore diameter of not less thann10 µm to not more than 500 µm and pore length of not lessnthan 20 µm to not more than 1 cm being juxtaposedlynarranged n obtained by a production process comprising rapidnfreeze-drying as a key technology. n Further, the invention relates to the above 3-dimensionalnporous scaffold in which seeding properties ofncells are improved by a pore enlargement treatment of onenside face by a separation operation, a salt elutionnoperation of a surface part, or a combination of thesenoperations. It becomes possible to produce a 3-dimensionalncell combination having excellent degree of tissuenformation and medical treatment effect by seeding a cell ornprecursor cell derived from a tissue in this scaffold, andnculturing them in an artificial environment and/or thenliving body. |