http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-1536023-B1
Outgoing Links
Predicate | Object |
---|---|
assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_4b8dff54c5d515dd011a9cf2700ea426 |
classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6834 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6837 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6816 |
classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6834 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6837 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6816 |
filingDate | 1999-12-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2008-03-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7e5b54a7fa03548193da1b371ce86518 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ed1f16de0c09210f89bdd5636ce1b84c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4aed0b9eed34abb080ae9e3795aa6c3f http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ad375ec6221b871afead7e22fba314d0 |
publicationDate | 2008-03-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | EP-1536023-B1 |
titleOfInvention | Methods, kits and compositions for the identification of nucleic acids electrostatically bound to matrices |
abstract | A method is provided for the detection or quantitation of a target sequence of a nucleic acid molecule in a sample, comprising: a) contacting the sample with a non-nucleotide probe which will hybridise to at least a portion of the target sequence to form a non-nucleotide probe/ target sequence complex; b) contacting the sample with a matrix wherein said nucleic acid molecule will electrostatically bind to the matrix and wherein the backbone of the non-nucleotide probe or probes is sufficiently neutral or positively charged, under electrostatic binding conditions, that it exhibits little or no affinity for the matrix; c) contacting the sample with an enzyme capable of degrading sample contaminants, including the nucleic acid molecule but not the non-nucleotide probe/target sequence complex, at a time after performing step (a); and d) detecting or quantitating the non-nucleotide probe or the non-nucleotide probe/target sequence complex at a time after performing step (b), as a means to detect or quantitate the target sequence in the sample. <??>A kit and a composition for use in such a method are also provided. |
priorityDate | 1998-12-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 142.