http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-1533367-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_de099a936149aa5beadaeb4d354a5802 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2509-00 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0623 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-0797 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-02 |
| filingDate | 2000-02-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_56529cfe26faacfe84e6fb3d51c348fa |
| publicationDate | 2005-05-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | EP-1533367-A1 |
| titleOfInvention | Use of collagenase in the preparation of neural stem cell cultures |
| abstract | The present invention discloses a method for the in vitro proliferation of a neural stemncell culture, which method excludes the proliferation of human embryonic stem cells,nsaid method comprising the steps of: (a) culturing a cell suspension containing one ornmore multipotent neural stem cells capable of producing progeny that are capable ofndifferentiating into neurons and glia in a culture medium containing at least onenproliferation-inducing growth factor to proliferate the neural stem cells to generate anneural stem cell culture; and (b) passaging the cell culture in (a) by treating the culturenwith an amount of a collagenase preparation effective to dissociate the cells in the culturenand passing the cell culture to a culture medium containing at least one proliferation-inducingngrowth factor to further proliferate the neural stem cell culture. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-1900810-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2008031957-A3 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2008031957-A2 |
| priorityDate | 1999-02-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 37.