http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-1531677-A2
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_7238585b2281c29b646487da046d9353 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-117 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2502-243 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2502-094 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-39 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-33 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-385 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2502-1114 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2503-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2502-1121 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0697 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-071 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 |
| filingDate | 2003-05-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a88edbee9e8f33245b26ff282989b341 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_04ee924df8070dc35008c17791117983 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_15c1a25bd299ef7e0fe8bd73654b80cf |
| publicationDate | 2005-05-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | EP-1531677-A2 |
| titleOfInvention | Three dimensional vaginal tissue model containing immune cells |
| abstract | Disclosed is a cervico-vaginal tissue equivalent comprised of vaginal epithelial cells and immune cells, cultured at the air-liquid interface. The tissue equivalent is capable of being infected with a sexually transmitted pathogen such as a virus (e.g., HIV), a bacteria, a helminthic parasite, or a fungus. The tissue equivalent is also capable of undergoing an allergic-type reaction or an irritant-type reaction. The tissue equivalent is characterized as having nucleated basal layer cells and nucleated suprabasal layer cells, and further as having cell layers external to the suprabasal layer progressively increasing in glycogen content and progressively decreasing in nuclei content. Immune cells of the tissue equivalent are primarily located in the basal and suprabasal layers. Also disclosed are methods for producing the tissue equivalent. The methods involve providing vaginal epithelial cells and immune cells, seeding the cells onto a porous support, and co culturing the seeded cells at the air-liquid interface under conditions appropriate for differentiation. One such method disclosed is for generation of the tissue equivalent in serum free medium. Specific cells from which the tissue equivalent is generated, and also specific preferred components of the medium in which the tissue equivalent is generated are provided. Also disclosed is a cervico-vaginal tissue equivalent produced by the methods disclosed herein. |
| priorityDate | 2002-06-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
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