| abstract |
This invention relates to a novel polyphosphate:AMPnphosphotransferase (PAP), a gene coding this PAP, and theirnuse. The PAP has the following properties:n (A) action: catalyzing of the following two reactions:n NMP + PolyP (n) → NDP + PolyP (n-1) n dNMP + PolyP (n) → dNDP + PolyP (n-1) n(wherein NMP represents nucleoside monophosphate, NDPnrepresents nucleoside diphosphate, dNMP representsndeoxynucleoside monophosphate, dNDP representsndeoxynucleoside diphosphate, n represents degree ofnpolymerization of the polyphosphate which is an integer ofnup to 100); (B) substrate specificity: specific to AMP, GMP, IMP,ndAMP, and dGMP, also acting with CMP, UMP, dCMP, and TMP; (C) molecular weight: about 55 to 56 Kd (kilodalton);nand (D) specific activity: at least 70 units per 1 mg ofnenzyme protein, |