http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-1512742-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_bd9cc964bd4d0311b94ff6058dd4723d http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_2caddbc1a4badfee661cf715d68e9c80 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_0e24390522c0d714e711ea7a1162ca33 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2310-14 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2310-111 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2320-50 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2330-30 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2310-53 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K48-00 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-111 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-79 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K48-00 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-79 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-54 |
| filingDate | 2003-09-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_26fa6bd262d2657d9b38416cb19e9514 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f245fc016da54eca3733d5ce82ed29ba http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_103647453f0b1e8fd9284d79f83c7b6c |
| publicationDate | 2005-03-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | EP-1512742-A1 |
| titleOfInvention | Conditional modification of sirnas expression |
| abstract | The present invention provides an in vitro and in vivo method for modulating the expression of a target gene in a conditional manner, utilizing small interfering (si) RNA. It also provides non-eukaryotic RNA polymerase expression constructs and siRNA expression constructs suitable for said method, a kit comprising said expression cassettes as well as the use of said expression cassettes for the preparation of medicaments for the treatment of various diseases associated with dysfunctional gene expression. <??>By functionally fusing the nucleotide sequence of a non-eukaryotic-RNA-polymerase (N-EU-Pol) with eukaryotic transcription regulating sequences, an induced cell-type specific or otherwise regulated expression of the foreign RNA polymerase in eukaryots becomes possible. Since the non-eukaryotic-RNA polymerase lacks a nucleic signal peptide, it will remain in the cytoplasm after translation. Thus, it is only able to transcribe target sequences cytoplasmatically, and the nucleus-bound functions such as the addition of a cap-structure or of a polyA-tail will not take place. siRNA coding sequences, which are functionally linked with a recognition sequence for the non-eukaryotic-RNA-polymerase will be transcribed in the cytoplasm by the non-eukaryotic-RNA-polymerase, resulting in non-capped and non- polyadenylated siRNAs. Since the expression of the non-eukaryotic-RNA-polymerase cell-type specific or otherwise regulated, the expression of the siRNA is accordingly also cell-type specific or otherwise regu-Iated. The functional link between coding sequences for siRNA with a recognition sequence for a conditionally regulated and expressed non-eukaryotic foreign-RNA polymerase will finally result in an intracellularly located, conditionally directed synthesis and function of siRNA in eukaryotic cells. <IMAGE> |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-4019636-A1 |
| priorityDate | 2003-09-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 245.