http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-1507456-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_1e0c9decd89e0ec3c2815631e084dd40 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-37 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-505 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-573 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-37 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-50 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-573 |
| filingDate | 2003-01-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3774c51ed56a07b4f1a7b0bad0a2d5c5 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b9d236ad78848dd3e49b88409fd4f5fc http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_faf0fb25f067f3151808699528285a1e http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f4c12fa9d1fa728669a717fc5f6661fc http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e16c51697edb6dfc193ce4b5beb5eef2 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d95c78e155718f8ba50061b740956bfb http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6e5f0351015c4f3df5793b4eff62c000 |
| publicationDate | 2005-02-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | EP-1507456-A1 |
| titleOfInvention | Visualization and quantitiation of cellular cytotoxicity using cell-permeable fluorogenic protease substrates and caspase activity indicator markers |
| abstract | This invention provides a non-radioactive assay to monitor and quantify the target-cell killing activities mediated by cytotoxic T lymphocytes (CTLs). This assay is predicated on the discovery that apoptosis pathway activation and, in particular, caspase activity, provides a measure of cytotoxic effector cell activity. In one embodiment, measurement of CTL-induced caspase activation in target cells is achieved through detection of the specific cleavage of fluorogenic caspase substrates. This assay reliably detects antigen-specific CTL killing of target cells, and provides a more sensitive, more informative and safer alternative to the standard 51Cr-release assay most often used to quantify CTL responses. The assay can be used to study CTL-mediated killing of primary host target cells of different cell lineages, and enables the study of antigen-specific cellular immune responses in real time at the single-cell level. As such, the assay can provide a valuable tool for studies of infectious disease pathogenesis and development of new vaccines and immunotherapies. |
| priorityDate | 2002-01-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 390.