| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_430c89d97f6839f5364904e710af9704 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6806 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N1-06
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1003
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6806
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6844 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-06 |
| filingDate |
2003-05-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3d5291ab083293a7ebec7a4e2583bacf
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_83bf5be02e4e7629fd7ef82bab73ecd6 |
| publicationDate |
2005-02-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
EP-1506995-A1 |
| titleOfInvention |
Method of effecting lysis of acid-fast bacteria and method of performing gene amplification or detection therewith |
| abstract |
A method of effecting lysis of acid-fast bacteria, comprising heating acid-fast bacteria in a liquid containing a non-ionicnsurfactant at a temperature of below the boiling point of the liquid. This method enables accomplishing secure lysis of acid-fastnbacteria in a simple manner within a short period of time without the use of special apparatus and agent and enables extracting genes.nThe heating is preferably conducted at 96°C for 10 min. As the nonionic surfactant use can be made of a d-sorbitol fatty acid ester,na polyoxyethylene glycol sorbitan alkyl ester, a polyoxyethylene glycol p-t-octylphenyl ether or the like. The pH value of the liquidnis preferably 8, and the liquid preferably contains EDTA. It is also preferred that before the heating, the acid-fast bacteria be treatednwith lipase. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2011028887-A2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10323267-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10190152-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11434519-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-2473596-A4
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10435735-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-2473596-A2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/AU-2010289430-B2 |
| priorityDate |
2002-05-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |