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filingDate 2003-05-07-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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publicationDate 2005-02-09-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber EP-1504259-A1
titleOfInvention Method for the determination of changes in a cellular morphological parameter
abstract Disclosed is method for measuring a change in a cellular morphological parameter, for example, the position and/or the shape of a cell. The method comprises labelling a population of cells with a marker substance which is in a non-detectable state, but which may be caused to become detectable by exposure to light of a suitable first wavelength. By exposing a sub-population of labelled cells to light of an appropriate first wavelength in a predetermined pattern, the sub-population of cells may be defined in which the area covered by the sub-population conforms to the pattern of illumination used. Subsequent examination of at least a portion of the population of cells using illuminating light of a second wavelength selected to be suitable for detection of the marker, will reveal patterns of distribution of the marker substance indicative of a change in said cellular morphological parameter. Also described and claimed is a method for screening for a test agent whose effect on the cellular morphological parameter is to be determined.
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