http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-1497312-A4
Outgoing Links
Predicate | Object |
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assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_a490020fd2580d5f04cbe4ea55b65c3f |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-68 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-483 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-78 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N27-447 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-50 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
filingDate | 2003-04-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_680dfeeb93b61e9433548f45e44c1bdf http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a5a54571b69460cd0b36950e217c2f78 |
publicationDate | 2005-05-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | EP-1497312-A4 |
titleOfInvention | SEPARATION AND DETECTION OF NUCLEIC ACIDS BY ELECTROPHORESIS WITH HIGH-AFFINITY ANTI-MIGRATORY INTERCALIMENT MARKER |
abstract | The invention provides novel electrophoretic methods for high-resolution separation and high-sensitivity detection of nucleic acids. The methods involve the use of a high-resolution buffer and a counter-migrating high-affinity intercalating dye in an electrophoresis system to achieve superior separation and detection of nucleic acids. A kit for separation and detection of nucleic acids in a sample by electrophoresis is also provided. The kit comprises a gel buffer containing a high-resolution intercalating dye with a resolution of at least 2.0 between the 271-bp and 282-bp ζX 171 HAIII nucleic acid fragments; and a high-affinity intercalating dye having a positive charge and DNA affinity constant of at least 106M-1. |
priorityDate | 2002-04-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 75.