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filingDate 2002-03-26-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f2c31d7020b026f6453291f7a13d917d
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publicationDate 2005-01-05-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber EP-1492878-A1
titleOfInvention Process for the preparation of a desired erythropoietin glyco-isoform profile
abstract The present invention provides a process for the production of erythropoietin (EPO) with high purity and with a desired profile of EPO glycol-isoforms by using a combination of specific chromatographic steps in such a manner that the starting EPO glycol-isoform profile is changed or modified. The applied chromatographic steps includes at least (a) dye affinity chromatography, and (b) hydrophobic chromatography and/or (c) anion-exchange chromatography. In a preferred embodiment, the process further includes (d) gel filtration chromatography. The present invention also provides a process for the determination of erythropoietin (EPO) glycol-isoform profile in an EPO containing composition.
isCitedBy http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2010034442-A1
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