http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-1490514-A4
Outgoing Links
Predicate | Object |
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assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_6dd45aa981988e937877aa9ed1069370 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6804 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6865 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-53 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N37-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-08 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 |
filingDate | 2003-03-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_349ca160053e0487c2cdd0621d0aec36 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_fe51d0c9fd9c631fdf46f74a4a706400 |
publicationDate | 2008-12-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | EP-1490514-A4 |
titleOfInvention | THROUGH ISOTHERME SINGLE PRIMER NUCLEIC ACID AMPLIFICATION IMPROVED DETECTION AND QUANTIFICATION OF ANALYTES |
abstract | The present invention provides novel methods of indirect analyte detection and quantification through amplification of oligonucleotide template attached to binding partners for analytes by nucleic acid amplification utilizing isothermal, single primer linear nucleic acid amplification methods. Methods of binding of binding partner that is attached to an oligonucleotide template to analyte, then amplifying at least a portion of the oligonucleotide template using a composite primer, primer extension, strand displacement, and optionally a termination sequence, are provided. Methods for amplifying sense RNA using a composite primer, primer extension, strand displacement, optionally template switching, a propromoter oligonucleotide and transcription are also provided. Methods for detecting and quantifying amplification products are also provided. The invention further provides compositions and kits for practicing said methods. |
priorityDate | 2002-03-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 232.