abstract |
The invention provides a method for selecting, from a repertoire ofnpolypeptides, a population of functional polypeptides which bind a target ligand in anfirst binding site and a generic ligand in a second binding site , which generic ligandnis capable of binding functional members of the repertoire regardless of target ligandnspecificity, comprising the steps of: a) contacting the repertoire with the genericnligand and selecting functional polypeptides bound thereto; and b) contacting thenselected functional polypeptides with the target ligand and selecting a population ofnpolypeptides which bind to the target ligand. The invention accordingly provides anmethod by which a polypeptide repertoire is preselected, according to functionality asndetermined by the ability to bind the generic ligand, and the subset of polypeptidesnobtained as a result of such preselection is then employed for further selectionnaccording to the ability to bind the target ligand. |