http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-1478776-A2
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_cd0d10fca42b67cdbcba1374cbc631fa |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6813 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate | 2003-02-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2f279bba7f86499272acafc0dfd6e32c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_17017f350b2ab7ab9ba3078949ee4e37 |
| publicationDate | 2004-11-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | EP-1478776-A2 |
| titleOfInvention | Method for the qualitative and quantitative determination of genetic material |
| abstract | The invention relates to a method for the qualitative and quantitative determination of genetic material that contains a known target sequence characteristic of the material and that can be present in addition to or mixed with another genetic material. According to the invention, a suspension that contains the genetic material with the target sequence and optionally other genetic material is (1) mixed with a molar excess of a first oligonucleotide probe whose nucleotide sequence is complementary to the target sequence, and optionally (2) mixed with a molar excess of a second oligonucleotide probe whose nucleotide sequence is complementary to a nucleotide sequence that is contained in all components of the entire genetic material to which the quantification shall apply. The entire genetic material, as far as double-stranded, is then converted to single strands, the first oligonucleotide probe is hybridized to the target sequence and optionally the second oligonucleotide probe is hybridized to the nucleotide sequence that is contained in all components of the genetic material. The concentration(s) of the non-hybridized first and optionally second oligonucleotide probe in the suspension is then determined and the share of genetic material having the target sequence in the entire genetic material to which the quantification shall be applied is determined from the concentration(s). |
| priorityDate | 2002-02-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 29.