| abstract |
Disclosing a method for screening a proteinninteractive with PPAR in a ligand-dependent manner, worksnas a useful tool for screening a drug ameliorating insulinnresistance. By the method, ECHLP as a main action ligand-dependentnPPAR binding molecule, FLJ13111 as a main actionnligand-selective factor interactive with PPARγ and AOP2 asnan adverse action ligand-dependent PPAR binding moleculenwere obtained. By using ECHLP interactive with PPAR,nFLJ13111 interactive with PPAR and AOP2 interactive withnPPAR, a screening system for a drug ameliorating insulinnresistance is constructed and disclosed, the drug givingnselectively the main action with no occurrence of thenadverse action. Additionally, a method for producing anpharmaceutical composition for ameliorating insulinnresistance is disclosed, which contains as the activencomponent, a promoting agent of the main action throughnPPAR, an agonist specific to the main action through PPAR,nan inhibitor of ECHLP interactive with PPAR to promote thenmain action through PPAR, a substance suppressing thenadverse action through PPARγ, an inhibitor of AOP2ninteractive with PPAR to suppress the adverse actionnthrough PPARγ, an activating agent of FLJ13111 interactivenwith PPAR to promote the main action through PPAR or annactivator of FLJ13111 expression. |