http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-1468105-A2
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_806f0a3a49d26f0b2efb79a3ea184ce9 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-63 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-67 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P21-02 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-67 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-63 |
| filingDate | 2003-01-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4450acc0fd45462ebee3f340fcd21193 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8fe8526bc470be1c82535a4a8123c2c9 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5ee951ea466385bbaa55f4cee8b10678 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f21960636e75c213c7ff116add8fd02c |
| publicationDate | 2004-10-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | EP-1468105-A2 |
| titleOfInvention | Recombinant protein expression |
| abstract | There are provided methods for the expression of a recombinant protein of interest, said methods comprising, in additional to various additional steps: a) culturing a host cell which expresses: i) one or more genes encoding the recombinant protein(s) of interest; ii) at least two genes encoding proteins selected from the group consisting of the chaperone proteins GroEL, GRoES, Dnak, DnaJ, GRpe, ClpB and their homologs (for example, Hsp104, Ydj1 and Ssal in yeast); under conditions suitable for protein expression; and separating said recombinant protein of interest from the host cell culture. Also provided are methods for increasing the degree of refolding of a recombinant protein of interest by ading a composition containing a chaperone protein to a preparation of the recombinant protein of interest in vitro. |
| priorityDate | 2002-01-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
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