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filingDate 2002-11-22-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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publicationDate 2004-10-06-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber EP-1463828-A1
titleOfInvention Method of detection and enumeration of bacteria
abstract A rapid method for detecting spoilage of a food sample, particularly a fish sample, by detecting and enumerating sulfide-producing bacteria (SPB). A medium containing iron and sulfur is combined with the food sample forming an incubation mixture which is incubated for a period of time. A plurality of fluorescence measurements are taken during an incubation period of about 4 hours to 17 hours at 300C. SPB are determined to be present in the sample if the fluorescence measurement initially increases and then decreases to form a fluorescence maximum (peak). The detection time of the fluorescence peak can be used with a correlation schedule to enumerate the SPB in the food sample. A visual test can also be used to identify color changes in the incubation mixture to provide a semi-quantitative enumeration of SPB effective in about 4 to 17 hours at 300C. Figure (1) is a graph depicting results for rapid enumeration of S. putrefaciens in pure cell cultures.
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