| abstract |
A scalable process and device for producing a biomolecule, in particularnpharmaceutical grade plasmid DNA. The process includes the steps of alkalinenlysis and a neutralization. For separating the lysate and the precipitate, the mixturenis allowed to gently flow downward through a clarification reactor that is partiallynfilled, in its lower part, with retention material like glass beads, whereby thenprecipitate is retained on top of and within the retention. In a preferred embodimentnof the lysis step, cell suspension and alkaline lysis solution flow through a lysisnreactor that is filled with particulate material like glass beads. The process can benrun continuosly and fully automated. |