http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-1444365-A4
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_3e3d4de2bbafe894539c7eddef26dedf |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-686 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-34 |
| filingDate | 2002-10-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_78386619fc8c2991bb3c1a05faed3ef8 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e93a9380e3c62263ad9cf16215312d68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ca5aa04f627532074dfa214ebfa15625 |
| publicationDate | 2005-07-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | EP-1444365-A4 |
| titleOfInvention | ASYMMETRIC PCR WITH NUCLEASE-FREE POLYMERASE OR NUCLEASERESISTENT MOLECULAR BEACONS |
| abstract | The invention provides methods for performing combined asymmetric amplification (e.g. asymmetric PCR amplification) and detection of nucleic acid targets using molecular beacons to detect the products. Methods using a polymerase having reduced or eliminated 5' to 3' nuclease activity are provided, as are methods using nuclease-resistant molecular beacons. Asymmetric amplifications using nuclease-free polymerase or nucleease-resistant molecular beacons provide dramatic improvements in signal intensity detected as a result of molecular beacon binding to a target nucleic acid, e.g., during asymmetric PCR. Attendant compositions, systems, devices and kits are also features of the invention. |
| priorityDate | 2001-10-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 178.