| abstract |
The present invention provides an animal model withnoverexpression of regucalcin that overexpresses regucalcin,nwhich is inherently expressed in the liver and the like of thenhigher animal, showing bone pathology typified by osteoporosis.nRegucalcin cDNA is cloned from rat liver cDNA library, and cDNAnencoding the full-length of regucalcin protein is isolated.nORF is cut from said rat regucalcin full length cDNA andnintroduced into an expression vector (pCXN2). Said genenexpression vector is microinjected to male pronucleus of thenfertilized egg of rat. Said fertilized egg is tranplanted intonthe uterine tube of a host rat to generate rats. Thus,nhomozygous rats are constructed from said generated rats. Saidntransgenic rats show remarkable bone pathology,nmorphologically as well as biochemically, and the body weightngain is suppressed significantly. |