| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_d5cd84f92e5e2ff95dfd19e4e329db3b |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6827
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6832
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-686
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6858 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-34
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H21-04
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 |
| filingDate |
2003-09-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5470256abc1a171d5cfd90ecef62577b
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_11c137bae3bd4b129132f291d6c85a0a
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ce317497a522919848934b6e46362416 |
| publicationDate |
2004-06-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
EP-1428892-A1 |
| titleOfInvention |
Dual-purpose primers and probes for providing enhanced hybridization assays by disruption of secondary structure formation |
| abstract |
The present invention provides primers and probes to be used in a method ofnenhancing hybridization of a probe to a target nucleotide sequence when the targetnsequence is capable of forming intramolecular secondary structures that interfere withnhybridization of the probe to the target sequence. In particular, the inventionnincludes a primer for amplifying a target nucleotide sequence, wherein at least anportion of the target nucleotide sequence can form an intramolecular secondarynstructure. The primer of the invention includes a primer nucleotide sequencencomplementary to a portion of the target nucleotide sequence that does not form ansecondary structure, and a blocking sequence substantially complementary to at leastna portion of the secondary structure-forming region of the amplified target nucleotidensequence, wherein the blocking sequence hybridizes to a portion of the secondarynstructure-forming region of the amplified target nucleotide sequence and blocks thenformation of the secondary structure. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8604182-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7927798-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8986931-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8288522-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8632970-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8426578-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8628918-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9663822-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-3743517-A4
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-1880025-A2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-1880025-A4
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7803541-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7709198-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8017360-B2 |
| priorityDate |
2002-09-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |