http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-1427851-A2
Outgoing Links
Predicate | Object |
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assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_280d87031f0c5786de1808713a1b76b7 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6869 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6816 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P19-34 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 |
filingDate | 2002-08-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_661e4fa2fc21da293224a704f16a743a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b018688c858e62dc9b21520cb8137ab6 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_70d376a14f32f41caec2189a7f70a42a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d9467003955b238e9aea0addd5557491 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3e31b0d6f074a4114fb1e974aa928d0c |
publicationDate | 2004-06-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | EP-1427851-A2 |
titleOfInvention | Method of determining the nucleotide sequence of oligonucleotides and dna molecules |
abstract | The present invention relates to a novel method for analyzing nucleic acid sequences based on real-time detection of DNA polymerase-catalyzed incorporation of each of the four nucleotide bases, supplied individually and serially in a microfluidic system, to a reaction cell containing a template system comprising a DNA fragment of unknown sequence and an oligonucleotide primer. Incorporation of a nucleotide base into the template system can be detected by any of a variety of methods including but not limited to fluorescence and chemiluminescence detection. Alternatively, microcalorimetic detection of the heat generated by the incorporation of a nucleotide into the extending template system using thermopile, thermistor and refractive index measurements can be used to detect extension reactions. |
priorityDate | 2001-08-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 645.