http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-1417332-A2
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_b46078e1950079a1dca5338ffc287e26 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6806 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-101 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1013 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1003 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6806 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 |
| filingDate | 2001-12-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6d4c6089f412b1cddf06c71678892e43 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a303f877b632ceb25f17977afecf314e |
| publicationDate | 2004-05-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | EP-1417332-A2 |
| titleOfInvention | Nucleic acid extraction method and kit |
| abstract | The present invention relates to a method and a kit for isolating nucleic acids from tissue samples. In particular, but not exclusively, formalin fixed paraffin embedded tissue samples. The isolated nucleic acids will normally be for use in a polymerase chain reaction (PCR). The inventive method comprises the sequential steps of: a) placing in a container the tissue sample, and a wax, a detergent and a tissue digestion enzyme; b) heating the container to a first temperature; c) raising the temperature of the container to a second temperature; d) cooling the container; e) forming a waxy-layer containing impurities and a nucleic acid containing layer; f) removing the waxy-layer, such that the nucleic acids are usable in a polymerase chain reaction. Kits are also provided for performing the same. |
| priorityDate | 2000-12-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 534.