| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_adb0e564374fedf34b36103d36e6455a |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1031
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-80
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-102
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P41-006
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P13-04
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1027 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-80
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P13-04
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P41-00 |
| filingDate |
2002-07-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d1f70d9813a314aa4475b3caa5d86b1a
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_dd43447d19789f17c4fdc7b434bef0c5 |
| publicationDate |
2004-04-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
EP-1409667-A2 |
| titleOfInvention |
Process for preparing variant polynucleotides |
| abstract |
The present invention discloses a process for the preparation of variant polynucleotides using a combination of mutagenesis of a starting population of polynucleotides and recombination of the mutated polynucleotides. The process comprises the steps of subjecting a population of polynucleotides to (a series of) two (or more) separate PCR's comprising a first PCR with a forward mutation-specific primer for a position to be mutated and a reverse universal primer and a second PCR with a forward universal primer and a reverse mutation-specific primer for a position to be mutated. The products of the (series of) two (or more) PCR's are assembled by a polymerase, preferably in one tube. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-2123768-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-2123767-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8383346-B2 |
| priorityDate |
2001-07-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |