http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-1403385-A1
Outgoing Links
Predicate | Object |
---|---|
assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_43a72884e25aaecffebe0942132d704f |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-706 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-70 |
filingDate | 2002-09-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e90234cfba9b02fb151e790316a4074e http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8ca8ae285aa1c85fc325e371cd304e6e http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_621c332c6f2ed61cb02178f612ea58e5 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b918bb8eb3e6d770247dd6dc35453601 |
publicationDate | 2004-03-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | EP-1403385-A1 |
titleOfInvention | Method for assaying replication of HBV and testing susceptibility to drugs |
abstract | A method for measuring the replication capacity of HBV, e.g. HBV present in a biological sample, possibly in the presence of a pharmaceutical product, in particular an antiviral agent, the method comprising:n (a) possibly extracting nucleic acids contained in the biological sample; (b) PCR amplification of HBV nucleic acids using at least two primer pairs to obtain at least two amplified HBV genomic fragments representing more-than-full-length HBV genome; (c) cloning the fragments obtained under (b) into a vector, so producing a vector containing more-than-full-length HBV DNA; (d) transfecting or transducing susceptible cells with the vector; (e) culturing the transfected or transduced cells in conditions allowing synthesis of HBV pgRNA from the cloned HBV DNA; (f) possibly treating the cultured cells with the pharmaceutical product, in particular antiviral agent; and (g) determination of the replication capacity of the HBV, possibly incidence of the pharmaceutical product, in particular antiviral agent, on viral gene expression and/or viral replication. |
isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2006090262-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-114204358-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-114204358-B http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-1696040-A1 |
priorityDate | 2002-09-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 162.