| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_bc426a01888806141dea4a42e37ef7da
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_ebf5692fb4f487e63af28a2c4846a187
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_242f1bcc60d97c7438495ec0058c0849 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6809 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6809
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-02
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 |
| filingDate |
2002-05-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7bbc08bc3c6b00fa3a474e13863e3ca6
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_44e15e2008010e5895100f8eca3d7357
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_92f329f2dc1dc1a4e10d3868ba4b8ecb
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f1d449cbda45f5cb7b5d0a08c0089664 |
| publicationDate |
2004-03-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
EP-1402072-A1 |
| titleOfInvention |
Accurate and efficient quantification of dna sensitivity by real-time pcr |
| abstract |
The sensitivity of regions of genomic DNA from chromatin to DNA-modifying agents are quantitated using real time PCR with careful use of reference regions and standard curves for improved accuracy over previous methods. A wide variety of DNA modifying agents are used to modify DNA, including single strand breakers, which allows analysis of a wider variety of DNA structures with greater precision than was previously possible. Methods also are provided that allow fine detailed analysis of hypersensitive DNA regions from as little as 100 cells. These methods also potentiate the investigation of more fine details of chromatin fiber folding and nucleosome-nucleosome contact. |
| priorityDate |
2001-05-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |