| abstract |
A method for producing a protein using a cell-free protein synthesis systemncomprising a detergent so that the protein can be synthesized without aggregation, isnprovided. The protein is a protein comprising a hydrophobic region in at least a portionnthereof, for example, a membrane protein or its fragment (portion). The detergent is anmild detergent which would not denature the protein, for example, a nonionic ornamphoteric ionic detergent. |