| abstract |
A coated substrate for immobilizing a biological substance etc. at highndensity with low background value. The substrate has a cross-linking reagentnrepresented by general formula A-L-B (wherein, A and B represent an identical orndifferent group which reacts with an active group of the coupling agent comprising annactive group, selected from an active ester group, isothiocyanate group, isocyanatengroup, imidazole group, carbodiimide group or aldehyde group, and wherein L is anlinking group linking A and B, selected from a straight chain alkyl group, aryl group,nallyl group or alkyl group having an amide group) bound thereonto by means of ancoupling agent comprising an active group. An biological substance or the like isnimmobilized on the substrate. |