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filingDate 2002-05-03-04:00^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 2003-12-17-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5aa006321f68d72bdaafa6f727e36ae5
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publicationDate 2003-12-17-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber EP-1359415-B1
titleOfInvention Device and process to lyse cells
abstract A platform disc (1) rotates about its center (2) and is depressed to form a chamber (3) having a volume of 0.1-100 microl. A second, otherwise similar chamber (4), is offset radially, further from the center than the first. A channel (5) connects them, carrying fluid between them under the influence of centripetal force during rotation. A platform disc (1) rotates about its center (2) and is depressed to form a chamber (3) having a volume of 0.1-100 microl. A second, otherwise similar chamber (4), is offset radially, further from the center than the first. A channel (5) connects them, carrying fluid between them under the influence of centripetal force during rotation. The first chamber includes a filter material located so that sample fluid introduced, is forced towards and/or out from the filter material and in the direction of the channel. Reagent causing lysis of biological cells is included on the filter material in re-soluble or removable form. The filter material retains cell membrane particles arising from cell lysis in the first chamber (3), during platform rotation. A retention device prevents or obstructs transport between chambers (3, 4), when the disc is stationary or rotates at a speed below a given limit. At most, 20 % of the fluid remains in the first chamber after rotation for a set interval at set speed. An Independent claim is included for the corresponding method.
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