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filingDate 2001-12-28-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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publicationDate 2006-02-01-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber EP-1357126-A4
titleOfInvention METHOD FOR DETERMINING LYSOPHOSPHOLIPASE D ACTIVITY
abstract Lysophospholipids represented by the following general formula (I): (I) wherein R1 and R2 are different from each other and one of them represents a saturated or unsaturated C8-22 fatty acid residue while the other represents hydrogen or acetyl; and X is a chromophore; a method of assaying lysophospholipase D activity characterized by using such a phospholipid as a substrate; a method of screening a substance inhibiting lysophospholipase D activity; and reagents and reagents kits useful in these methods. Use of these novel lysophospholipids as a substrate makes it possible to specifically assay lysophospholipase D activity at a high sensitivity. Moreover, use of a chromophore which is safe and can be easily detected makes it possible to assay a large number of samples quickly.
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