| abstract |
A method of labelling molecules which includes providing in ancommon medium a label molecule, a marker ligand able to bindna member of a specific binding pair, such as an antigen, ansbp member, an enzyme able to catalyse binding of the labelnmolecule to other molecules, the enzyme being associated withnthe marker ligand; causing or allowing binding of the markernligand to the sbp member; and causing or allowing binding ofnthe label molecule to other molecules in the vicinity of thenmarker ligand bound to the sbp member. The marker ligand maynbe an antibody or any specific binding molecule, such as anchemokine or cytokine. A complementary member of thenspecific binding pair may be included, e.g. an antibody, or andiverse population of such sbp members, e.g. antibodies, maynbe included within which those which bind the counterpart sbpnmember, e.g. antigen, may be labelled and subsequentlynisolated for manipulation and/or use. Suitable labelsninclude biotin-tyramine with signal transfer being catalysednby hydrogen peroxidase. Cells, virus particles and othernmoieties may be labelled, for identification or obtention ofnproteins which interact or are in close proximity with anparticular sbp member, or of cells of interest, or fornenhancement of labelling, e.g. for cell sorting. |