| abstract |
A mutated expandase enzyme having higher activity on penicillin G isnprovided'to produce 7-aminodeacetoxycephalosporanic acid (7=ADCA), whichnmutated expandase enzyme has one or more amino acid substitutions selected fromnmethionine 73, glycine 79, valine 275, leucine 277, cysteine 281, glycine 300,nasparagine 304, isoleucine 305, threonine 91, alanine 106, cysteine 155, tyrosine 184,nmethionine 188 and histidine 244, provided that the amino acid substitution at thenresidue position of asparagine 304 is not N304L and the amino acid substitution atnthe residue position of cysteine 155 is C155Y. |