| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_c4b06fec68c364d15c457fa09a86e404 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y02A50-30
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K39-00 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-445
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-815 |
| classificationIPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K39-00 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K39-015
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-68
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-81
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-445
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-30
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-10 |
| filingDate |
2001-12-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d6a5ad93603481c26832db94f6781b1e
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a42388a50806de5bb66a12a1756e2b8e
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1a5cc3cbdc10d0a0bd9aa383cd650af9
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1f8e238717fa30a783f655bf94204d7c
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8a8b18714473b714f455d1554ecd69b6 |
| publicationDate |
2003-09-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
EP-1346049-A2 |
| titleOfInvention |
EFFICIENT EXPRESSION OF i PLASMODIUM /i APICAL MEMBRANE ANTIGEN 1 IN YEAST CELLS |
| abstract |
The present invention provides a means of efficient expression of Plasmodium AMA-1 ectodomain or a functional part, derivative and/or analogue thereof in a eukaryotic expression system. Preferably, said Plasmodium AMA-1 ectodomain is Pf AMA-1 ectodomain. This protein is preferably expressed in yeast, more preferably in Pichia pastoris. Efficient expression is possible using a method for producing mRNA encoding said Plasmodium AMA-1 ectodomain in a yeast cell, comprising providing said yeast cell with a nucleic acid encoding said Plasmodium AMA-1 ectodomain, said nucleic acid being modified to utilize said yeast's codon usage. Preferably, at least one putative yeast polyadenylation consensus sequence in said nucleic acid has been modified. More preferably, also at least one site in said protein that is generally glycosylated by eukaryotic expression systems, has been removed. |
| priorityDate |
2000-12-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |