http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-1332208-A2
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_f7ec6e3cecafcf6df7050fcb6a17c35b |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6853 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6844 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6853 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6844 |
| filingDate | 2001-10-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_addf5dfd7cd578034bfe7e3fe8fd9a3e http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e385cc0d6e6fd4878198a69fba2a49e7 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_98f5ea7d5b03f69a26cc396275fcaa12 |
| publicationDate | 2003-08-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | EP-1332208-A2 |
| titleOfInvention | Partially double-stranded nucleic acids, methods of making, and use thereof |
| abstract | A process is disclosed for generating at least one partially double-stranded target nucleic acid, which contains at least one single-stranded region at a tenninal end. The process comprises the steps of (a) providing at least one primer, P1, containing at least one labile nucleotide; (b) combining at least one target nucleic acid sequence with P1 to generate a double-stranded polynucleotide containing at least one labile nucleotide; (c) exposing the double-stranded polynucleotide to conditions that promote single-strand cleavage of the polynucleotide at the site of the at least one labile nucleotide of primer P1; and (d) exposing the cleaved polynucleotide to conditions that promote the dissociation of the cleaved portions of primer PI from a terminal end. The labile nucleotide may be dUTP, wherein the single-stranded cleavage of the polynucleotide at the site of the labile nucleotide occurs by treatment with uracil N-glycosylase. |
| priorityDate | 2000-10-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 508.