http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-1326992-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_d202e9e866984fe229f6facc38f1d740 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2510-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2710-10343 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-64 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-86 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-64 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N7-01 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-861 |
| filingDate | 2001-10-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6e76c2805949d1a02a4d636010d6ca6c |
| publicationDate | 2003-07-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | EP-1326992-A1 |
| titleOfInvention | Method for circularizing adenoviral nucleic acid via homologous recombination |
| abstract | Applicants have identified a process which exploits the bacterial homologous recombination system to convert double-stranded linear adenovirus genome into circularized plasmid form (See Figure 4). The system functions via adenoviral terminal fragments present on the plasmid that are less than 500 basepairs each. The result is a plasmid which is more readily analyzed by restriction digestion, PCR, DNA sequencing or used in transient transfection studies. The adenovirus plasmids that are generated can be rescued back into virus form. The entire procedure takes 4 days or less instead of the weeks required of plaque purification or dilution cloning isolation techniques. An additional plus of the instant invention is that the disclosed method does not require the use of tissue culture materials or facilities. The disclosed method allows for a more extensive and thorough examination of a viral preparation, in that it allows for the detection of variants incapable of propagation without the assistance of co-infecting intact adenoviral genomes. Under standard conditions of plaque purification, these variant genomes are not detected. It is predicted that far more variant genomes will be observed using the rapid method than would otherwise be detected by standard plaque purification methods. |
| priorityDate | 2000-10-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 46.